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Zocoler,Analice Martins Daleffi; Sanches,Andréia Cristina Conegero; Albrecht,Ingrid; Mello,João Carlos Palazzo de. |
The extract from stem bark of Stryphnodendron obovatum Benth. was chromatographed on a Sephadex® LH-20 column, and yielded nine compounds: gallic acid (GA), p-hydroxybenzoic acid (PHB), gallocatechin (GC), epigallocatechin (EPG), 4'-O-methylgallocatechin (MGC), epigallocatechin-(4β→8)-epigallocatechin (EPEP), epigallocatechin-(4β→8)-gallocatechin (EPGC), robinetinidol-(4α→8)-gallocatechin (ROGC) and robinetinidol-(4β→8)-epigallocatechin (ROEP). Evaluation of the antioxidant capacity in vitro by the methods of DPPH free radical (IC50; μg/mL) and reduction of the phosphomolybdenum complex (RAC) gave the following results, respectively: crude extract 4.52 and 0.8242; ethyl-acetate fraction 4.04 and 0.9537; aqueous fraction 5.58 and 0.9275. The crude extract... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stryphnodendron obovatum; Antioxidant capacity; Condensed tannins; DPPH free radical; Phosphomolybdenum complex. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300009 |
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Lopes,Gisely Cristiny; Sanches,Andréia Cristina Conegero; Toledo,Cleyton Eduardo Mendes de; Isler,Ana Cristina; Mello,João Carlos Palazzo de. |
Um método de separação e quantificação por cromatografia líquida de alta eficiência (CLAE) em fase reversa foi desenvolvido usando água (0,05% de TFA):acetonitrila (0,05% de TFA) como fase móvel, em sistema gradiente para a análise dos flavan-3-óis presentes em extrato semipurificado das cascas de Stryphnodendron adstringens, Stryphnodendron polyphyllum e Stryphnodendron obovatum. A CLAE foi realizada com a fração acetato de etila (FAE) sendo submetida à extração em fase sólida (cartucho C18-E) com metanol:água (2:8), filtrada por membrana de porosidade 0,5 μm; a pré-coluna e coluna empregadas foram Phenomenex® Gemini C-18 (5 μm), com esta última mantida a 30 ºC, com vazão de 0,8 mL/min e detecção a 210 nm. Utilizaram-se soluções dos padrões de ácido... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stryphnodendron adstringens; Stryphnodendron polyphyllum; Stryphnodendron obovatum; Cromatografia líquida de alta eficiência; Taninos/determinação quantitativa; Análise quantitativa. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100017 |
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Otobone,Fernanda Jacques; Sanches,Andréia Conegero; Nagae,Rosangela Lika; Martins,Juliana Vanessa Colombo; Obici,Simoni; Mello,João Carlos Palazzo de; Audi,Elisabeth Aparecida. |
The effect of crude lyophilized extract (EBPC) and the semi-purified constituents (EPA and EPB) of Paullinia cupana (guaraná) seeds long-term administered in rats by gavage at different doses was studied on cognitive behavior in rats. EBPC (30.0 mg/kg) and EPA (2.0 mg/kg), but not EPB (2.0 or 4.0 mg/kg) showed a smaller escape latency to find the emerged platform in Morris water maze test (MWMT), showing nootropic-like effect in normal rats, and in scopolamine induced amnesia rats compared to their controls (saline + 0.2% Tween 80) group. These extracts had no significant effect in open field test (OFT). Caffeine did alter escape latency in MWMT only in scopolamine induced amnesia rats and increased the crossings number in OFT, showing significant... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cognitive effects; Guaraná; Morris water maze; Paullinia cupana. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600007 |
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Ferreira,Magda Rhayanny Assunção; Santiago,Rosilene Rodrigues; Silva-Rocha,Walicyranison Plínio; Souza,Luanda Barbara Ferreira Canário de; Faria,Maria Graciela Icher; Mello,João Carlos Palazzo de; Langassner,Silvana Maria Zucolotto; Chaves,Guilherme Maranhão; Milan,Eveline Pipolo; Svidzinski,Terezinha Inez Estivalet; Soares,Luiz Alberto Lira. |
The aim of this study was evaluate the in vitro antifungal activity of crude extracts from Eugenia uniflora, Libidibia ferrea and Psidium guajava. The extracts were obtained by turbo-extraction using water (AQ) or acetone-water (AC-W) (7:3, v/v) as solvents and lyophilized to obtain the crude extracts (CE). The CE were characterized by UV-Vis, TLC and HPLC. The activity of CEs was investigated against clinical isolates of Candida spp. and the Minimum Inhibitory Concentration (MIC), MIC50 and MIC90 were determinated. The analysis by TLC showed that all CEs presented polyphenols (flavonoids and tannins). The CEs from E. uniflora showed higher amount of polyphenols (30.35 ± 2.15%, AC-W) and the HPLC analysis revealed the tannins in all extracts. The CEs of E.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicinal plants; Herbal extracts; Polyphenols; Candida isolates; Oral candidiasis. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100608 |
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Galvão,Suzana Maria Pereira; Mendes,Fúlvio Rieli; Oliveira,Maria Gabriela Menezes de; Mattei,Rita; Mello,João Carlos Palazzo de; Roman Júnior,Walter Antonio; Carlini,Elisaldo de Araújo. |
Few data exists about the pharmacological properties of Heteropterys aphrodisiaca O. Mach. (Malpighiaceae), which is native to the scrubland regions of Brazil. The present study investigated the effects of oral treatment with H. aphrodisiaca extract (BST0298) on the learning and memory of young (3-6 months) and aged (21-23 months) rats, and compared the in vitro antioxidant activity of three lots collected in different years. An improvement in the number of sessions to learn the task was observed in the left/right discrimination test in aged rats treated for 45 days with 25 mg/kg (7.0 ± 0.5; p=0.005) or 50 mg/kg (7.6 ± 0.6; p=0.012) compared with control old rats (11.0 ± 1.6). On the other hand, pre-treatment did not improve the performance of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heteropterys aphrodisiaca O. Mach/pharmacognosy; Heteropterys aphrodisiaca O. Mach/antioxidant activity; Malpighiaceae/pharmacognosy; Antioxidants; Flavonoids; Plant extract. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400020 |
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Migliato,Ketylin Fernanda; Carvalho,Elisângela Simões de; Sacramento,Luis Vitor Silva do; Mello,João Carlos Palazzo de; Baby,André Rolim; Velasco,Maria Valéria Robles; Salgado,Hérida Regina Nunes. |
A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, μg mL-1) and limit of quantification (LOQ, μg mL-1). The visible spectrophotometric method presented linearity (r² = 0.9979 ± 0.0010) over the concentration range 0.25-7.5 μg mL-1 of standard pyrogallic acid, precision < 2.918171%, recovery/accuracy ranging from 96.228693 to 107.17701%, LOD = 0.21 μg... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Total polyphenols; Syzygium cumini; Botanical extract; Analytical method validation; Spectrophotometry. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100015 |
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